Evaluation of acute oral toxicity of zinc oxide nanoparticles in rats

Zinc oxide nanoparticles (ZnO-NPs) are widely used in the food and fertilizer industries. During our previous study, bean fertilized by two doses (20 40 ppm) of ZnO-NPs with a particle size 20 nm was investigated on rats. Therefore, this experiment designed to evaluate effect that were administrated orally rats once day at ppm. Hematological biochemical parameters quantified addition investigation deleterious liver kidney histopathological. In-vitro cytotoxicity Vero cell line also studied. Eighteen male Wistar divided into three groups. Normal control (G1) administered deionized water (1ml/rat). Groups G2 G3 received 1ml/rat suspensions ppm ZnO-NPs, respectively. Hematology showed mean corpuscular hemoglobin concentration (MCHC) lower G3. White blood count decreased G2. had an elevated hematocrit. In G3, plasma glucose while uric acid alkaline phosphatase activity increased. Plasma creatinine, aspartate aminotransferase, alanine creatine kinase all albumin levels decreased. Treatment for 72 h resulted no toxicity cells when compared normal ZnO. Biochemical data confirmed histopathology. conclusion, both minimal effects hematological parameters. While should be applied carefully due their negative functions.